Fasting Blood Measurements and Hormone Assays

Fasting blood samples (10 ml) were obtained after a 12-hour overnight fasting. Serum and plasma isolations were done using centrifugation at 4500 rpm at 4 °C for 10 min immediately. Then, aliquots were frozen at − 80 °C until use. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), and serum glucose concentrations were measured using a commercial kit (Pars Azmoon, Tehran, Iran). Serum low-density lipoprotein cholesterol (LDL-C) was calculated by Friedewald’s equation using TC, TG, and HDL-C concentrations [35 (link)]. In addition, enzyme-linked immunosorbent assay (ELISA) kits were used to detect insulin levels. Homeostasis Model Assessment-Insulin Resistance Index (HOMA-IR) and Quantitative Insulin Sensitivity Check Index (QUICKI) were calculated according to the specified formula [36 (link), 37 (link)]. Moreover, plasma Agouti-Related Peptide (Ag-RP) and α-Melanocyte-Stimulating Hormone (α-MSH) were assayed using ELISA kits (Bioassay Technology Laboratory, China).

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Mahmoudinezhad M., Khosravaniardakani S., Saljoughi Badelou L., Fayyazishishavan E., Kahroba H, & Farhangi M.A. (2023). The integrative panel of fatty acid desaturase-2 (FADS2) rs174583 gene polymorphism and dietary indices (DQI-I and HEI) affects cardiovascular risk factors among obese individuals. BMC Endocrine Disorders, 23, 41.

Publication 2023

ELISA kits

Agouti Bioassay Blood Centrifugation Cholesterol Enzyme linked immunosorbent assay Frozen Glucose High density lipoprotein cholesterol Homeostasis Insulin Insulin resistance Insulin sensitivity Isolations Low density lipoprotein cholesterol Pars Peptide Plasma Serum Triglyceride α melanocyte stimulating hormone

Corresponding Organization : Tabriz University of Medical Sciences

Other organizations : Florida International University, Shahid Beheshti University of Medical Sciences, The University of Texas Health Science Center at Houston, Maastricht University

Top 5 similar protocols

Variable analysis

independent variables

Fasting status (12-hour overnight fasting)

dependent variables

Total cholesterol (TC)
High-density lipoprotein cholesterol (HDL-C)
Triglyceride (TG)
Serum glucose concentrations
Serum low-density lipoprotein cholesterol (LDL-C)
Insulin levels
Homeostasis Model Assessment-Insulin Resistance Index (HOMA-IR)
Quantitative Insulin Sensitivity Check Index (QUICKI)
Plasma Agouti-Related Peptide (Ag-RP)
Plasma α-Melanocyte-Stimulating Hormone (α-MSH)

control variables

Sample collection (blood samples obtained under standardized conditions: 10 ml, 12-hour overnight fasting)
Sample processing (serum and plasma isolated using centrifugation at 4500 rpm, 4 °C, 10 min)
Sample storage (aliquots frozen at -80 °C until use)
Biochemical analysis (commercial kits and ELISA used for measurements)

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