Virulence Factor Production in Bacteria

The production of the virulence factors caseinase, gelatinase, lipase, β-hemolysin, and phospholipase was determined as described by Natrah et al. [30 (link)]. For the lipase and phospholipase assays, marine agar 2216 (MA, Becton-Dickinson) plates supplemented with 1% Tween 80 (Sigma-Aldrich, Darmstadt, Germany) and 1% egg yolk emulsion (Oxoid, Hampshire, United Kingdom), respectively, were used. The caseinase and gelatinase assays were performed using plates with double-strength MA supplemented with a 4% skim milk powder suspension (Oxoid) and plates with MA with 0.5% gelatin (Sigma-Aldrich) added, respectively. The β-hemolysis of sheep erythrocytes was determined using Columbia Blood agar (Oxoid). All assays were performed in triplicate.

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Urtubia R., Miranda C.D., Rodríguez S., Dubert J., Barja J.L, & Rojas R. (2023). First Report, Characterization and Pathogenicity of Vibrio chagasii Isolated from Diseased Reared Larvae of Chilean Scallop, Argopecten purpuratus (Lamarck, 1819). Pathogens, 12(2), 183.

Publication 2023

Tween 80 egg yolk emulsion gelatin Columbia Blood agar

Agar Assays Blood Caseinase Egg yolk Emulsion Erythrocytes Gelatin Gelatinase Hemolysin Hemolysis Lipase Marine Milk Phospholipase Powder Sheep Tween 80 Virulence factors

Corresponding Organization :

Other organizations : Universidad Católica del Norte, Universidade de Santiago de Compostela

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Production of the virulence factors caseinase
Production of the virulence factors gelatinase
Production of the virulence factors lipase
Production of the virulence factors β-hemolysin
Production of the virulence factors phospholipase

control variables

Marine agar 2216 (MA, Becton-Dickinson) plates
1% Tween 80 (Sigma-Aldrich, Darmstadt, Germany)
1% egg yolk emulsion (Oxoid, Hampshire, United Kingdom)
Double-strength MA supplemented with a 4% skim milk powder suspension (Oxoid)
MA with 0.5% gelatin (Sigma-Aldrich) added
Columbia Blood agar (Oxoid)

controls

Positive control: None mentioned
Negative control: None mentioned

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