Macrophages were seeded in Permanox Lab-Tek chamber slides (177,429, Nunc) and then fixed with 2.5% glutaraldehyde (18,426, Ted Pella, Redding, CA, USA) in 0.1 M of phosphate buffer, pH 7.4 (S369 and S37, Fisher Scientific) containing 0.1 M of sucrose (S2-500, Fisher Scientific). Campus Microscopy & Imaging Facility at The Ohio State University performed sample processing as previously described (Krause et al., 2018a (link)). FEI Tecnai G2 Spirit transmission electron microscope plus AMT camera system was used for taking images.
Badr A., Eltobgy M., Krause K., Hamilton K., Estfanous S., Daily K.P., Abu Khweek A., Hegazi A., Anne M.N., Carafice C., Robledo-Avila F., Saqr Y., Zhang X., Bonfield T.L., Gavrilin M.A., Partida-Sanchez S., Seveau S., Cormet-Boyaka E, & Amer A.O. (2022). CFTR Modulators Restore Acidification of Autophago-Lysosomes and Bacterial Clearance in Cystic Fibrosis Macrophages. Frontiers in Cellular and Infection Microbiology, 12, 819554.
Buffer Glutaraldehyde MacrophagesMicroscopy PhosphateSucrose Transmission electron microscope
Corresponding Organization : The Ohio State University
Other organizations :
Mansoura University, Max Planck Unit for the Science of Pathogens, Helwan University, Birzeit University, Nationwide Children's Hospital, Case Western Reserve University
Macrophages were seeded in Permanox Lab-Tek chamber slides
dependent variables
Macrophages were fixed with 2.5% glutaraldehyde in 0.1 M of phosphate buffer, pH 7.4 containing 0.1 M of sucrose
Macrophage samples were imaged using an FEI Tecnai G2 Spirit transmission electron microscope plus AMT camera system
control variables
0.1 M of phosphate buffer, pH 7.4
0.1 M of sucrose
controls
No positive or negative controls were explicitly mentioned in the provided information.
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