This methodology has been described previously (14 (link)). In brief, adult Wistar rats were sacrificed without perfusion, and brains were removed, midsagittally sectioned, fixed by immersion in 4% paraformaldehyde for 1 hour at 4 C°, cryopreserved in 40% sucrose for 48 hours at 4°C, embedded in freezing compound media, and snap frozen in isopentane chilled with liquid nitrogen. Seven micron-thick sections were then incubated with 0.3% hydrogen peroxide for 20 minutes, with 10% goat serum in phosphate-buffered saline for 1 hour, and then labeled with patient or comparison sample (dilution 1:200) at 4°C overnight. The next day, sections were washed and then incubated with a secondary biotinylated goat antihuman IgG (dilution 1:2,000, Vector BA-3000 [Burlingame, Calif., Vector Laboratories]) for 1 hour at room temperature, and the reactivity was developed with the avidin-biotin-peroxidase method (Burlingame, Calif., Vector Laboratories).