Plasma was collected from heparinised whole blood and stored at −80°C until use. HCMV infection status was determined by HCMV IgG ELISA (BioKit). IgG antibodies to pertussis toxin (PT; NIBSC) and to formalin-inactivated whole H1N1 influenza virus (influenza A/California/7/2006(H1N1)v(NYMC-X179A); H1N1; NIBSC) were determined using in-house ELISA assays with goat anti-human IgG-peroxidase (Sigma) as the secondary antibody and SIGMAFAST™ OPD (Sigma) as the substrate. IgG concentrations were calculated by interpolation from a standard curve which was produced using anti-pertussis reference serum (NIBSC; IU/ml) or using plasma from a donor with high titres of antibodies to H1N1 influenza (IgG concentration expressed in Arbitrary ELISA Units, AEU) [28 (link)]). The pooled AB plasma used for in vitro assays contained 6.8 IU/ml IgG to PT and had an H1N1 titre of 273.8 AEU.
Protocol detail
HCMV and Influenza Antibody Measurement
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Anti igg
Antibodies
Antibodies igg
Antibody
Assays
Blood
Donor
Elisa
Formalin
Goat
H1n1 influenza virus
Hcmv
Hcmv infection
Human
Influenza
Peroxidase
Pertussis
Pertussis toxin
Plasma
Serum
Corresponding Organization :
Other organizations : University of London, London School of Hygiene & Tropical Medicine
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Protocol cited in 1 other protocol
Variable analysis
independent variables
- HCMV infection status
- IgG antibodies to pertussis toxin (PT)
- IgG antibodies to formalin-inactivated whole H1N1 influenza virus (influenza A/California/7/2006(H1N1)v(NYMC-X179A); H1N1)
- Plasma was collected from heparinised whole blood and stored at −80°C until use
- Goat anti-human IgG-peroxidase (Sigma) as the secondary antibody
- SIGMAFAST™ OPD (Sigma) as the substrate
- Anti-pertussis reference serum (NIBSC; IU/ml)
- Plasma from a donor with high titres of antibodies to H1N1 influenza (IgG concentration expressed in Arbitrary ELISA Units, AEU)
- Pooled AB plasma used for in vitro assays contained 6.8 IU/ml IgG to PT and had an H1N1 titre of 273.8 AEU
- Plasma from a donor with high titres of antibodies to H1N1 influenza
- Pooled AB plasma used for in vitro assays contained 6.8 IU/ml IgG to PT and had an H1N1 titre of 273.8 AEU
- Not explicitly mentioned
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