Scaffolds were prepared from the microspheres using our previously established technology [33 (link), 22 (link), 27 (link), 35 (link), 37 (link), 38 (link)]. In brief, lyophilized microspheres (50–70 mg) were dispersed in DI H2O and loaded into a syringe. The dispersion was then pumped using a programmable syringe pump (PHD 22/2000; Harvard Apparatus, Inc., Holliston, MA) into a cylindrical plastic mold (diameter ~ 4 mm) having a filter at the bottom until a height of about 2 mm was reached. The scaffolds were 3.8–4.0 mm in diameter and around 2 mm in height. The packed microspheres were then sintered with ethanol-acetone (95:5 v/v) for 55 min. The scaffolds were further lyophilized for 48 h and sterilized with ethylene oxide for 12 h prior to cell seeding experiments. A total of four different groups were tested in the study and were named according to the composition of microspheres as: BLANK, BMP, TH73 or TCP/HAp 7:3, and TH11 or TCP/HAp 1:1.
Protocol detail
Fabrication of Sintered Microsphere Scaffolds
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Other organizations : University of Kansas
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Variable analysis
independent variables
- Composition of microspheres
- Sintering time (55 min)
- Scaffold properties (diameter, height)
- Cell seeding experiments
- Lyophilized microsphere amount (50-70 mg)
- Syringe pump parameters
- Cylindrical mold diameter (~ 4 mm)
- Scaffold height (~ 2 mm)
- Ethanol-acetone (95:5 v/v) sintering
- Lyophilization duration (48 h)
- Ethylene oxide sterilization (12 h)
- Positive control: Not specified
- Negative control: BLANK group
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