Cloning FAT1 and ZNF750 ORFs

The open reading frame (ORF) of human FAT1 and ZNF750 transcripts were generously provided by Timothy Chan's group from Memorial Sloan-Kettering Cancer Center^{27 (link)} and Paul Khavari's group from Veterans Affairs Palo Alto Healthcare System^{24 (link)}, respectively. Both ORFs were sub-cloned into lentiviral based expression vector SHC003 (Sigma-Aldrich) using Nhe I and Fse I cloning sites. SHC003-Turbo-GFP was used as control (Sigma-Aldrich). The lentiviral based shRNA vectors (shXPO1 and shFAT2 sequences are listed in Supplementary Table 13b) were generated with PLKO.1 backbone (Sigma-Aldrich) using Age I and EcoR I cloning sites. SHC002-Scramble shRNA was used as control (Sigma-Aldrich). The cloning primers are listed in Supplementary Table 13c.

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Lin D.C., Hao J.J., Nagata Y., Xu L., Shang L., Meng X., Sato Y., Okuno Y., Varela A.M., Ding L.W., Garg M., Liu L.Z., Yang H., Yin D., Shi Z.Z., Jiang Y.Y., Gu W.Y., Gong T., Zhang Y., Xu X., Kalid O., Shacham S., Ogawa S., Wang M.R, & Koeffler H.P. (2014). Genomic and molecular characterization of esophageal squamous cell carcinoma. Nature genetics, 46(5), 467-473.

Publication 2014

SHC003 SHC003-Turbo-GFP PLKO.1 backbone SHC002-Scramble shRNA

Backbone Cancer Human Orfs Primers Shrna Vectors Veterans

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

Other organizations : The University of Tokyo, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Karyopharm Therapeutics (United States), National University Hospital

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Protocol cited in 3 other protocols

Variable analysis

independent variables

FAT1 and ZNF750 transcripts
ShXPO1 and shFAT2 sequences

dependent variables

Not explicitly mentioned

control variables

SHC003-Turbo-GFP
SHC002-Scramble shRNA

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