Comprehensive Western Blot and qPCR Protocol

Western blotting was performed as previously described^{2 (link)}. Primary and secondary antibodies used in this study are shown in Supplemental Table 1. For fluorescent antibodies membranes were imaged using Odyssey infrared imaging system (Li-Cor), and for HRP conjugated antibodies, membranes were treated with ECL reagents (Bio-Rad or Millipore Sigma) developed using autoradiography film (Genesee Scientific). Band intensities were quantified using ImageJ. RNA was extracted using the RNeasy kit (Qiagen). cDNA was synthesized using the ImProm-II Reverse Transcription System (Promega). Taqman probes used for qPCR are listed in Supplemental Table 1, and qPCR was performed using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). Actb and Gapdh were used for normalization controls. Reagent concentrations and supplier information are included in the Reagent Table.