Protocol detail

Protein Extraction from Mouse Tissues

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Normal buccal mucosa and tongue from 5 pairs of Tgfbr1^f/f/Pten^f/f and Tgfbr1/Pten 2cKO mice, together with tumors that developed in Tgfbr1/Pten 2cKO mice, were carefully dissected. A total of 40 μg of protein from each sample were denatured and then loaded in each lane of NuPAGE 4-12% Bis-Tris precast gel. Additional details are provided in the Supplementary Data.

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Bian Y., Hall B., Sun Z.J., Molinolo A., Chen W., Gutkind J.S., Van Waes C, & Kulkarni A.B. (2011). Loss of TGF-β Signaling and PTEN Promotes Head and Neck Squamous Cell Carcinoma through Cellular Senescence Evasion and Cancer-related Inflammation. Oncogene, 31(28), 3322-3332.

Publication 2011

A protein Bis tris Buccal mucosa Mice Pten Tgfbr1 Tongue Tumors

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Protocol cited in 9 other protocols

Variable analysis

independent variables

Genotype (Tgfbr1^f/f^/Pten^f/f^ and Tgfbr1/Pten 2cKO mice)

dependent variables

Protein expression (from normal buccal mucosa and tongue, and tumors that developed in Tgfbr1/Pten 2cKO mice)

control variables

Amount of protein loaded (40 μg per sample)
Tissue dissection (normal buccal mucosa and tongue from 5 pairs of mice)
Protein extraction and denaturation

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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