Kidney Histology and Immunostaining Analysis

Kidneys were harvested from CTL and FA-injected mice or human kidneys. Histological analysis was examined on formalin-fixed, paraffin-embedded kidney sections stained by PAS and Picrosirius red (Polyscience #24901). Immunofluorescence staining was performed with antibodies against JAG1 (Abcam #ab109536; 1:500) or NOTCH2 (Abcam #ab8926; 1:100). Tubule specific markers were used as previously described [18 (link),63 (link)]. Immunohistochemistry staining was performed with antibody against αSMA (Sigma #A5228; 1:500) and COX IV (Abcam # ab16056; 1:200) in paraffin-embedded kidney sections. For each section, five random fields were quantified in an unbiased manner using ImageJ.