All groups of rats were fed and maintained on a normal salt diet (NSD, 0.4% NaCl, Teklad, Harlan Laboratories, Madison, WI). Rats were fed a high-salt diet for 7 days (HSD, 4% NaCl, Teklad, Harlan Laboratories), or infused with AngII (AngII, 200 ng/kg/min (Moreno et al. 2003 (link)), Phoenix Pharmaceuticals, Burlingame, CA), or a combination of both. Osmotic mini-pumps containing AngII (model 2002; Alzet, Palo Alto, CA) were implanted subcutaneously under isoflurane anesthesia and sterile conditions according to the manufacturer's instructions as described previously in a subgroup of rats (Loria et al. 2010b (link)).
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