High-Salt Diet and Angiotensin II Infusion

All groups of rats were fed and maintained on a normal salt diet (NSD, 0.4% NaCl, Teklad, Harlan Laboratories, Madison, WI). Rats were fed a high-salt diet for 7 days (HSD, 4% NaCl, Teklad, Harlan Laboratories), or infused with AngII (AngII, 200 ng/kg/min (Moreno et al. 2003 (link)), Phoenix Pharmaceuticals, Burlingame, CA), or a combination of both. Osmotic mini-pumps containing AngII (model 2002; Alzet, Palo Alto, CA) were implanted subcutaneously under isoflurane anesthesia and sterile conditions according to the manufacturer's instructions as described previously in a subgroup of rats (Loria et al. 2010b (link)).

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Loria A.S., Pollock D.M, & Pollock J.S. (2015). Angiotensin II is required to induce exaggerated salt sensitivity in Dahl rats exposed to maternal separation. Physiological Reports, 3(5), e12408.

Publication 2015

Anesthesia Diet salt Isoflurane Nacl Osmotic Pharmaceuticals Rats Sterile

Corresponding Organization : Augusta University

Other organizations : University of Alabama at Birmingham

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Variable analysis

independent variables

Normal salt diet (NSD, 0.4% NaCl)
High-salt diet (HSD, 4% NaCl)
AngII infusion (200 ng/kg/min)
Combination of high-salt diet and AngII infusion

dependent variables

Not explicitly mentioned

control variables

All groups were fed and maintained on a normal salt diet (NSD, 0.4% NaCl) initially

controls

Normal salt diet (NSD, 0.4% NaCl) as a control condition

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