Genome Sequencing of L. monocytogenes Isolates from Food Samples

A total of 17 L. monocytogenes isolate species classified by Vitek MS (bioMerieux, Marcy-l’Étoile, France) were selected for the whole genome sequencing and genomic analyses. The strains came from a collection of the Department of Industrial and Food Microbiology at University of Warmia and Mazury in Olsztyn. Strains were isolated from food samples and food-processing premises (samples were taken from both non-food contact surfaces (drains, floors, freezers, aprons, door handles, and taps) and food contact surfaces) between 2018–2019 using the standard ISO 11290-1:2017-. The identification of the species level was conducted on MALDI-TOF MS (Vitek MS, biomerieux, France) and double confirmed by performing the standard PCR proposed by Ryu at al. [51 (link)]. The strains and sources of isolation are listed in Table 1.

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Zakrzewski A.J., Kurpas M., Zadernowska A., Chajęcka-Wierzchowska W, & Fraqueza M.J. (2023). A Comprehensive Virulence and Resistance Characteristics of Listeria monocytogenes Isolated from Fish and the Fish Industry Environment. International Journal of Molecular Sciences, 24(4), 3581.

Publication 2023

Vitek MS MALDI-TOF MS

Food Food microbiology Genomic Isolation Ms maldi Strains

Corresponding Organization : University of Warmia and Mazury in Olsztyn

Other organizations : Gdańsk Medical University, University of Lisbon

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Variable analysis

independent variables

Isolate species of L. monocytogenes

dependent variables

Whole genome sequencing and genomic analyses of the L. monocytogenes isolates

control variables

Food samples and food-processing premises (non-food contact surfaces and food contact surfaces) from where the L. monocytogenes isolates were collected between 2018-2019
Identification of the species level conducted on MALDI-TOF MS (Vitek MS, biomerieux, France) and confirmed by standard PCR proposed by Ryu et al. [51]

positive controls

Standard PCR proposed by Ryu et al. [51] used to confirm the identification of the L. monocytogenes isolates

negative controls

No negative controls were explicitly mentioned in the provided information

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