Quantifying Serum Hormones via ID-LC-MS/MS

Serum total T (TT) and E2 levels were measured using an isotope dilution high-performance liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) in the NHANES, while the concentrations of sex hormone-binding globulin (SHBG) were quantified using chemo-luminescence measurements of the reaction products via a photomultiplier tube after binding to SHBG with immuno-antibodies (30 (link)). According to previous literature, we only indirectly assessed the approximate amount of circulating free testosterone (FT) (31 (link)) and the activity of aromatase (32 (link)) through the free androgen index (FAI) that was calculated as the value of TT (ng/dL) divided by SHBG (nmol/L) and the ratio of TT to E2 (TT/E2), respectively. Bioavailable testosterone was calculated according to the Vermeulen et al. formula (33 (link)).

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Su X., Jin K., Zhou X., Zhang Z., Zhang C., Li Y., Yang M., Huang X., Xu S., Wei Q., Cheng X., Yang L, & Qiu S. (2023). The association between sex hormones and periodontitis among American adults: A cross-sectional study. Frontiers in Endocrinology, 14, 1125819.

Publication 2023

Androgen Antibodies Aromatase Dilution High performance liquid chromatography Isotope Luminescence measurements Ms ms Serum Sex hormone binding globulin Testosterone

Corresponding Organization : West China Hospital of Sichuan University

Other organizations : First Affiliated Hospital Zhejiang University

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Variable analysis

independent variables

Serum total T (TT) levels
Serum E2 levels

dependent variables

Concentrations of sex hormone-binding globulin (SHBG)
Approximate amount of circulating free testosterone (FT)
Activity of aromatase
Bioavailable testosterone

control variables

Not explicitly mentioned

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