Analyzing BnGR2R3-MYB Gene Promoters

The promoter regions of the BnGR2R3-MYB genes, 1,000 bp genomic DNA upstream sequences of each of the 105 BnGR2R3-MYB were selected, and the cis-elements predicted using PlantCare (http://bioinformatics.psb.ugent.be/web tools/plantcare/html/). Response class elements such as light-responsive, plant growth, stress-responsive and phytohormone-responsive were filtered and the cis-acting elements visualized in TBtools, and displayed with a heatmap. Following blasting in BLASTP against Swissport database, functional annotation of BnGMYB proteins was performed in Blast2GO Tool and subsequent mapping Gene Ontology (GO) terms, and visualization with R ggpolt2.

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Feng X., Abubakar A.S., Chen K., Yu C., Zhu A., Chen J., Gao G., Wang X., Mou P, & Chen P. (2023). Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes. Frontiers in Genetics, 14, 1080909.

Publication 2023

Dna sequences Genomic Light Myb genes Phytohormone Plant growth Proteins annotation Response elements

Corresponding Organization : Chinese Academy of Agricultural Sciences

Other organizations : Bayero University Kano

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Variable analysis

independent variables

Promoter regions of the BnGR2R3-MYB genes, 1,000 bp genomic DNA upstream sequences of each of the 105 BnGR2R3-MYB

dependent variables

Cis-elements predicted using PlantCare tools
Response class elements such as light-responsive, plant growth, stress-responsive and phytohormone-responsive
Functional annotation of BnGMYB proteins performed in Blast2GO Tool
Gene Ontology (GO) terms

control variables

Not explicitly mentioned

controls

No positive or negative controls were specified by the authors

Annotations

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