Combination Treatments for Cell Viability

Cytarabine (AraC) (77 (link)), trans-ISRIB (4 (link), 63 (link)), FX-1 (49 (link)), and toyocamycin (64 (link), 65 (link)) were obtained from Cayman Chemicals. FXR1 OE and control cells were treated with 1 μM trans-ISRIB for 24 hours. For cell viability that was measured by trypan blue staining and cell counts (1 (link)), FXR1 OE and control cells were treated individually or with a combination of 5 μM AraC and 1 μM trans-ISRIB for 24 hours, 100 nM toyocamycin and 1 μM AraC, and 10 μM FX-1 and 5 μM AraC.

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Datta C., Truesdell S.S., Wu K.Q., Bukhari S.I., Ngue H., Buchanan B., Le Tonqueze O., Lee S., Kollu S., Granovetter M.A., Boukhali M., Kreuzer J., Batool M.S., Balaj L., Haas W, & Vasudevan S. (2022). Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells. Science Advances, 8(43), eabo1304.

Publication 2022

trans-ISRIB toyocamycin

Arac Cayman Cell viability Cells Cytarabine Toyocamycin Trans isrib Trypan blue

Corresponding Organization : Harvard University

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Variable analysis

independent variables

Cytarabine (AraC)
Trans-ISRIB
Toyocamycin

dependent variables

Cell viability measured by trypan blue staining and cell counts

control variables

FXR1 OE and control cells

controls

FXR1 OE and control cells were treated with 1 μM trans-ISRIB for 24 hours.
FXR1 OE and control cells were treated individually or with a combination of 5 μM AraC and 1 μM trans-ISRIB for 24 hours, 100 nM toyocamycin and 1 μM AraC, and 10 μM FX-1 and 5 μM AraC.

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