The hippocampus was harvested 2 h after the behavioral analysis for the determination of HDAC2 (1:500; Abcam, UK), acetyl-H3K9 (1:2,000; Millipore, USA), acetyl-H4K12 (1:2,000;Millipore, USA), BDNF (1:500; Abcam, UK), TrkB (1:500; Abcam, UK), p-TrkB (1:1,000; Abcam, UK), p-CaMKII (1:500; Abcam, UK), p-CREB (1:200; Abcam, UK), caspase-3 (1:100; Abcam, UK), and anti-β-actin (1:1,000, Santa Cruz, USA) as previously described [19 (link)]. Bands were visualized by enhanced chemiluminescence and quantitated with the Image Quant Software (Syngene, Cambridge, UK).
Protocol detail
Hippocampal Molecular Analyses in Behavior
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Variable analysis
independent variables
- Time of hippocampus harvesting (2 h after behavioral analysis)
- HDAC2 (1:500; Abcam, UK)
- Acetyl-H3K9 (1:2,000; Millipore, USA)
- Acetyl-H4K12 (1:2,000;Millipore, USA)
- BDNF (1:500; Abcam, UK)
- TrkB (1:500; Abcam, UK)
- P-TrkB (1:1,000; Abcam, UK)
- P-CaMKII (1:500; Abcam, UK)
- P-CREB (1:200; Abcam, UK)
- Caspase-3 (1:100; Abcam, UK)
- Anti-β-actin (1:1,000, Santa Cruz, USA)
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