Pooled HUVECs (Lonza) were cultured in
EGM-2 MV medium (CC-3202, Lonza) and used for network formation between
passages 3 and 7. Normal human lung fibroblasts (NHLFs) (Lonza) were
cultured in FBM-2 medium (CC-3132, Lonza) and used for the experiments
between passages 5 and 12. The NHLFs were used here as Margolis et
al. reported that NHLFs can facilitate vessel network formation.42 (link) To construct the hierarchical vessel-supported
tumor model, the HCT116 cells (ATCC) were cultured in high-glucose
DMEM (Gibco) with 10% fetal bovine serum (R&D systems) and 1%
penicillin–streptomycin solution (Cytiva). All of the cells
were proliferated in 25 cm2 flasks, and the culture medium
was changed every other day.
EGM-2 MV medium (CC-3202, Lonza) and used for network formation between
passages 3 and 7. Normal human lung fibroblasts (NHLFs) (Lonza) were
cultured in FBM-2 medium (CC-3132, Lonza) and used for the experiments
between passages 5 and 12. The NHLFs were used here as Margolis et
al. reported that NHLFs can facilitate vessel network formation.42 (link) To construct the hierarchical vessel-supported
tumor model, the HCT116 cells (ATCC) were cultured in high-glucose
DMEM (Gibco) with 10% fetal bovine serum (R&D systems) and 1%
penicillin–streptomycin solution (Cytiva). All of the cells
were proliferated in 25 cm2 flasks, and the culture medium
was changed every other day.
