ELISA Assays for Complement Pathways
Corresponding Organization : Sanquin
Other organizations : University of Amsterdam, Emma Kinderziekenhuis, Radboud University Nijmegen, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Newcastle University, University of Bern, Luzerner Kantonsspital, National Institute for Public Health and the Environment
Variable analysis
- Serum type (NHS, FB-depleted, or FD-depleted)
- Purified FB (162.5 μg mL^-1) or FD (1.05 μg mL^-1) supplementation
- Anti-FB (202 μg mL^-1), anti-FD (1.3 μg mL^-1), or anti-C1q (31.75 μg mL^-1) antibody supplementation
- Complement activation measured by ELISA
- LPS coating concentration (40 μg mL^-1) for the alternative pathway ELISA
- Agglutinated human IgG (AHG) coating concentration (5 or 0.5 μg mL^-1) for the classical pathway ELISA
- Veronal Buffer composition (1.8 mM sodium barbital, 3.1 mM barbituric acid, pH 7.3-7.4) with 0.1% Tween-20, 0.3% BSA, 5 mM MgCl2, and 10 mM EDTA for the alternative pathway ELISA
- Veronal Buffer composition (1 mM CaCl2 and 0.5 mM MgCl2) for the classical pathway ELISA
- Biotinylated anti-C3.19 as the conjugate for ELISA development
- Streptavidin-HRP for the classical pathway ELISA development
- None specified
- None specified
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