Synapsin Antibody as Arthropod Neuroanatomical Marker

A monoclonal mouse anti-Drosophila synapsin antibody (“SYNORF1”, Developmental Studies Hybridoma Bank) raised against a Drosophila GST-synapsin fusion protein was applied. This antibody reacts with a highly conserved epitope, as it labels neuropil structures over a wide range of arthropod taxa (e.g. [64 (link),65 (link),66 (link),67 (link)]), including arachnid representatives, e.g., spiders [58 (link),68 (link),69 (link)], scorpions [49 (link),70 (link)], and amblypygids (whip spiders) [58 (link)]. This antibody has also been used as a structural marker in the focus species of this contribution, C. cancroides [49 (link)]. In Western blots of brain tissues of Drosophila and the crustacean Coenobita clypeatus identical bands were stained by the synapsin antibody, which suggests that the epitope for SYNORF1 is strongly conserved between Drosophila and Coenobita [64 (link)].

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Stemme T, & Pfeffer S.E. (2021). Anatomy of the Nervous System in Chelifer cancroides (Arachnida: Pseudoscorpiones) with a Distinct Sensory Pathway Associated with the Pedipalps. Insects, 13(1), 25.

Publication 2021

SYNORF1

Anti antibody Antibody Arachnid Arthropod Brain Crustacean Drosophila Drosophila synapsin protein Epitope Hybridoma Mouse Neuropil Scorpions Spiders Synapsin Tissues Western blots

Corresponding Organization : Universität Ulm

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Variable analysis

independent variables

Monoclonal mouse anti-Drosophila synapsin antibody ("SYNORF1", Developmental Studies Hybridoma Bank) raised against a Drosophila GST-synapsin fusion protein

dependent variables

Labeling of neuropil structures over a wide range of arthropod taxa, including arachnid representatives (spiders, scorpions, and amblypygids)

control variables

Not explicitly mentioned

controls

Positive control: Western blots of brain tissues of Drosophila and the crustacean Coenobita clypeatus, where identical bands were stained by the synapsin antibody, suggesting the epitope for SYNORF1 is strongly conserved between Drosophila and Coenobita.

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