The experiments were performed using C57BL/6J wild-type mice of the B6;Cg-Fgf-2tm1Zll strain [61 (link)]. Since the genetic background is not standardized, wild-type mice were generated by own breeding. Genotyping was performed as previously described [15 (link)].
Male mice (n = 20) were bred at Hannover Medical School (Germany) and kept in the same temperature- and humidity-controlled room on a 14 h light/10 h dark schedule and housed in open cages in groups of four to five with food and water available ad libitum. Starting one-week prior to the drinking experiment, animals were individually housed. The hygienic status was routinely monitored in accordance with the FELASA recommendations [62 (link)]. No evidence of infectious agents was revealed except for occasional positive tests for Rodentibacter pneumotropica and Helicobacter spp.
Male mice (n = 20) were bred at Hannover Medical School (Germany) and kept in the same temperature- and humidity-controlled room on a 14 h light/10 h dark schedule and housed in open cages in groups of four to five with food and water available ad libitum. Starting one-week prior to the drinking experiment, animals were individually housed. The hygienic status was routinely monitored in accordance with the FELASA recommendations [62 (link)]. No evidence of infectious agents was revealed except for occasional positive tests for Rodentibacter pneumotropica and Helicobacter spp.
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