Protocol detail

Scratch Wound Migration Assay for Transfected MCF-7 Cells

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Scratch wound migration assays were conducted using stably transfected MCF-7 cells. Confluent monolayers of transfected MCF-7 cells in 24-well plates were serum starved for 24 h to induce quiescence, and were then treated for 1 h with either serum free media, vehicle control, 70 nM UO126 (1,4-diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene; Abcam, Melbourne, VIC, Australia), or 1.5 nM PF-562271 (N-Methyl-N-[3-[[[2-[2-oxo-1,3-dihydroindol-5-yl)amimo]-5-(trifluoromethyl)pyrimidin-4-yl]amino)pyridine-2-yl)methanesulfonamide; Abcam). Following this, a scratch wound migration assay was performed as previously described (30 (link)).

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Birgersson M., Chi M., Miller C., Brzozowski J.S., Brown J., Schofield L., Taylor O.G., Pearsall E.A., Hewitt J., Gedye C., Lincz L.F, & Skelding K.A. (2021). A Novel Role for Brain and Acute Leukemia Cytoplasmic (BAALC) in Human Breast Cancer Metastasis. Frontiers in Oncology, 11, 656120.

Publication 2021

PF-562271

Butadiene Mcf 7 cells Methanesulfonamide Migration assay Pyridine Serum Serum free media Uo126 Wound

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Variable analysis

independent variables

Treatment with serum free media
Treatment with vehicle control
Treatment with 70 nM UO126
Treatment with 1.5 nM PF-562271

dependent variables

Scratch wound migration

control variables

Stably transfected MCF-7 cells
Confluent monolayers of transfected MCF-7 cells in 24-well plates
Serum starvation for 24 h to induce quiescence

controls

Positive control: Not explicitly mentioned
Negative control: Vehicle control

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