Skeletal Morphology Analysis in Mig-6 Mice

Whole-body scans were collected in 6-week-, 11-week-, 12-month-, and 18-month-old control and Mig-6^over/over male and female mice. Mice were euthanized and imaged using the General Electric (GE) SpeCZT microCT machine [45 (link)] at a resolution of 50 μm/voxel or 100 μm/voxel. GE Healthcare MicroView software (v2.2) was used to generate 2D maximum intensity projection and 3D isosurface images to evaluate skeletal morphology. MicroView was used to create a line measurement tool in order to calculate the bone lengths; femur lengths were calculated from the proximal point of the greater trochanter to the base of the lateral femoral condyle. Tibia lengths were measured from the midpoint medial plateau to the medial malleolus. Humerus lengths were measured from the midpoint of the greater tubercle to the center of the olecranon fossa.

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Bellini M., Pest M.A., Miranda-Rodrigues M., Qin L., Jeong J.W, & Beier F. (2020). Overexpression of MIG-6 in the cartilage induces an osteoarthritis-like phenotype in mice. Arthritis Research & Therapy, 22, 119.

Publication 2020

MicroView software (v2.2)

Bone Condyle Electric Female Femoral Greater trochanter Greater tubercle Humerus Male Mice Microct Olecranon fossa Skeletal Tibia Whole body scans

Corresponding Organization :

Other organizations : Western University, University of Pennsylvania, Michigan State University, Grand Rapids Community College

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Variable analysis

independent variables

Genotype (control vs. Mig-6over/over)
Sex (male vs. female)
Age (6 weeks, 11 weeks, 12 months, 18 months)

dependent variables

Bone length (femur, tibia, humerus)

control variables

Mouse strain
Imaging method (GE SpeCZT microCT machine)
Imaging resolution (50 μm/voxel or 100 μm/voxel)
Image analysis software (GE Healthcare MicroView)

controls

Positive control: Not specified
Negative control: control mice (non-Mig-6over/over)

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