Protocol detail

Immunohistochemical Analysis of HCC Recurrence

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Eighteen fresh tissue specimens of HCC and three fresh tissue of non-tumor adjacent tissue, as well as 162 paraffin-embedded HCC specimens were utilized, the detailed information was shown in Additional file 1: Table S1 The criteria for determining patient recurrence is that tumors is found in the liver, lung, skeleton, lymph and other positions after complete healing. These samples were collected during surgical procedures from patients with HCC according to a protocol approved by the institutional review board of the First Affiliated Hospital of Sun Yat-sen University. All patients provided written, informed consent for participation in the study and provision of tumor samples. IHC was performed according to our previous methods [23 (link), 24 (link)]. Anti-MCM3 antibody (ab4460, Abcam) was used. The images were captured using the AxioVision Rel.4.6 computerized image analysis system (Carl Zeiss Co Ltd., Jena, Germany).

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Meng W., Xie B., Yang Q., Jia C., Tang H., Zhang X., Zhang Y., Zhang J., Li H, & Fu B. (2019). Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-κB pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 263.

Publication 2019

ab4460 AxioVision Rel.4.6

Anti antibody Institutional review board Liver Lung Lymph Paraffin Patients Recurrence Skeleton Surgical procedures Tissue Tumor

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Variable analysis

independent variables

Eighteen fresh tissue specimens of HCC
Three fresh tissue of non-tumor adjacent tissue
162 paraffin-embedded HCC specimens

dependent variables

MCM3 expression (measured by IHC)

control variables

Tissues were collected from patients with HCC according to a protocol approved by the institutional review board
All patients provided written, informed consent for participation in the study and provision of tumor samples

controls

Positive control: None explicitly mentioned
Negative control: None explicitly mentioned

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