For immunofluorescent staining of γH2AX (Ser139) to observe DNA damage, the collected cells were reacted with anti-γH2AX antibody (Rabbit polyclonal, #11585573, Thermo Fisher Scientific) and Alexa Fluor 594-conjugated antibody (Rabbit polyclonal, #8889, Cell Signaling Technology Inc.). After counterstaining the nuclei using 6-diamidino-2-phenylindol (DAPI, Thermo Fisher Scientific Inc.), images were acquired [18 (link)].
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