Generating and Characterizing MmuPV1 Virus Stock

MmuPV1 virus stock was generated by isolating MmuPV1 virions from papillomas in nude mice as described previously [18 (link),19 (link),60 (link)]. The MmuPV1 infection model was established in nude mice using quasivirions generated (as described previously [19 (link),61 (link)]) using a clone of MmuPV1 obtained in the Lambert lab. Briefly, this clone of MmuPV1 was made by performing rolling circle amplification on the virus extracts (generously provided by Dr. Aravind Ingle, ACTREC, India) from the original colony of nude mice infected with MmuPV1 [15 (link)] followed by cloning into the pUC19 vector [15 (link),16 (link)]. To encapsidate MmuPV1 genome we used pMusSHELL, a Mammalian expression plasmid with codon modified L1 and L2 genes of MmuPV1 (generously provided by Dr. Chris Buck, NIH) [60 (link)]. To confirm that the virus stock was infectious, nude mice were infected in parallel during each experiment as positive controls.

Free full text: Click here

Uberoi A., Yoshida S., Frazer I.H., Pitot H.C, & Lambert P.F. (2016). Role of Ultraviolet Radiation in Papillomavirus-Induced Disease. PLoS Pathogens, 12(5), e1005664.

Publication 2016

Codon Genes Genome Infectious Mammalian Nude mice Papillomas Plasmid Vector Virions Virus

Corresponding Organization : Translational Research Institute

Top 5 similar protocols

Protocol cited in 11 other protocols

Variable analysis

independent variables

Isolation of MmuPV1 virions from papillomas in nude mice
Generation of MmuPV1 quasivirions using a clone of MmuPV1 obtained in the Lambert lab

dependent variables

Infectivity of the MmuPV1 virus stock

control variables

Nude mice infected in parallel during each experiment as positive controls

controls

Positive controls: Nude mice infected in parallel during each experiment

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!