Immunohistochemical Analysis of Xenograft Tumors

The endpoint xenograft tumors were isolated, fixed in formalin, and paraffin embedded. Tissue sections were processed for immunostaining and incubated overnight with p-AMPKα (T172) (40H9) (#2535, 1:200 dilution), Ki-67 (D2H10) (#9027, 1:200 dilution), or cleaved caspase-3 (Asp175) (5A1E) (#9664, 1:400 dilution) antibodies (Cell Signaling Technology). Next, the sections were counterstained with hematoxylin and subjected to IHC analysis as described previously (8 (link)).

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Hong J., Maacha S., Pidkovka N., Bates A., Salaria S.N., Washington M.K, & Belkhiri A. (2022). AXL Promotes Metformin-Induced Apoptosis Through Mediation of Autophagy by Activating ROS-AMPK-ULK1 Signaling in Human Esophageal Adenocarcinoma. Frontiers in Oncology, 12, 903874.

Publication 2022

Ki-67 (D2H10) cleaved caspase-3 (Asp175) (5A1E)

Antibodies Caspase 3 Dilution Formalin Hematoxylin Paraffin Tissue Tumors Xenograft

Corresponding Organization :

Other organizations : Vanderbilt University Medical Center, Qatar Airways (Qatar), South College

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Variable analysis

independent variables

Antibody treatment conditions: p-AMPKα (T172) (40H9) (#2535, 1:200 dilution), Ki-67 (D2H10) (#9027, 1:200 dilution), or cleaved caspase-3 (Asp175) (5A1E) (#9664, 1:400 dilution)

dependent variables

Immunostaining analysis of the endpoint xenograft tumors

control variables

Formalin fixation and paraffin embedding of the tissue samples
Hematoxylin counterstaining of the tissue sections

controls

No positive or negative controls were explicitly mentioned in the provided information.

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