RNA-seq and RT-PCR Analysis of Liver Transcriptome

Liver samples were used to obtain total RNA firstly. After checking RNA quality, they were consigned to Shanghai Personal Biotechnology Co., Ltd. for constructing RNA-seq libraries and sequencing using Illumina NovaSeq. In addition, extracted RNA was reverse transcribed into cDNA for verifying genes expression by RT-PCR method using SYBR Green Pro Taq™ II kit (Agbio, Hunan, China) on the FQD-96A (Bioer, Hangzhou, China). Primers were shown in Table S1. Detailed data processes such as differential expression genes identification, GO and KEGG pathways analysis and calculation of gene expression all referred to our previous report [25 (link), 26 (link)].