Lipid inhibitors regulate macrophage response

Compound C (Cayman Chemicals) was suspended in dimethyl sulfoxide (DMSO) at a stock concentration of 10 μM and added to J774A.1 macrophages at the indicated concentrations 1 h prior to bacterial inoculation. The Compound C dosage was determined based on data from previous studies (50 (link)). Compound C remained on macrophages throughout infection. The cytotoxicity of Compound C in J774A.1 macrophages was determined using a Vybrant 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay kit (Thermo Fisher) according to the manufacturer’s protocol. Atglistatin (30 μM) (Cayman Chemicals), triacsin C (10 μM) (Enzo Life Sciences), and T863 (10 μM) (Cayman Chemicals) were suspended in DMSO (12 (link), 30 (link)). For lipid inhibitor studies, we pretreated cells overnight with compounds and continued treatment throughout infection.

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Dominguez S.R., Whiles S., Deobald K.N, & Kawula T. (2022). Francisella tularensis Exploits AMPK Activation to Harvest Host-Derived Nutrients Liberated from Host Lipolysis. Infection and Immunity, 90(8), e00155-22.

Publication 2022

Compound C Atglistatin triacsin C

Assay Atglistatin Bacterial inoculation Bromide Cayman Cell proliferation Cells Cytotoxicity Dimethyl sulfoxide Diphenyl Infection Lipid Macrophages Tetrazolium Triacsin c

Corresponding Organization : Washington State University

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Variable analysis

independent variables

Compound C concentrations
Atglistatin (30 μM)
Triacsin C (10 μM)
T863 (10 μM)

dependent variables

Cell viability/cytotoxicity (measured using MTT assay)

control variables

Compound C (Cayman Chemicals) was suspended in dimethyl sulfoxide (DMSO) at a stock concentration of 10 μM
Compound C remained on macrophages throughout infection
Atglistatin, triacsin C, and T863 were suspended in DMSO

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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