In Vivo Quantification of Amyloid-Beta Using [11C]PiB PET

[¹¹C]Pittsburgh compound‐B ([¹¹C]PiB) was synthesized in accordance with previously described methods.
²⁸ (link) PET imaging was performed as described previously.
²¹ (link) Briefly, PET imaging was performed using a Siemens/CTI ECAT HR+ scanner (Siemens Medical Solutions, Knoxville, TN) operating in three‐dimensional (3D) imaging mode. Subjects were injected intravenously with 15 mCi of high specific activity [¹¹C]PiB (> 2 Ci/μmol at end‐of‐synthesis) before being positioned in the scanner. Following a 10–15 min transmission scan using rotating ⁶⁸Ge/⁶⁸Ga rod sources for the purpose of attenuation correction of PET emission data, a 20‐min emission scan was acquired (4 × 5 min frames) beginning 50 min after injection. PET emission data were reconstructed using 3D filtered back projection with Fourier rebinning, and standard corrections for photon attenuation, scatter, and radioactive decay were applied.

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Schweitzer N., Li J., Thurston R.C., Lopresti B., Klunk W.E., Snitz B., Tudorascu D., Cohen A., Kamboh M.I., Halligan‐Eddy E., Iordanova B., Villemagne V.L., Aizenstein H, & Wu M. (2023). Sex‐dependent alterations in hippocampal connectivity are linked to cerebrovascular and amyloid pathologies in normal aging. Alzheimer's & Dementia, 20(2), 914-924.

Publication 2023

Siemens/CTI ECAT HR+ scanner

Corresponding Organization :

Other organizations : University of Pittsburgh

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Variable analysis

independent variables

Injection of 15 mCi of high specific activity [11C]PiB (> 2 Ci/μmol at end-of-synthesis)

dependent variables

PET imaging data

control variables

10-15 min transmission scan using rotating 68Ge/68Ga rod sources for attenuation correction of PET emission data
20-min emission scan (4 × 5 min frames) beginning 50 min after injection
3D filtered back projection with Fourier rebinning
Standard corrections for photon attenuation, scatter, and radioactive decay

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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