Protein lysates were prepared as described previously [31 (link)]. Western blot analysis was performed with primary antibodies for GFP (sc-9996, 1:2000), Tau 46 (sc-32274, 1:500), and β-actin (sc-47778, 1:1000) were obtained from Santa Cruz Biotech (Santa Cruz, CA, USA).
Phospho-GSK-3α/β (Ser21/9, 9331s, 1:1000), Phosphor-(Ser/Thr) PKA Substrate (9621s, 1:1000), and Phosphor-Tau (Ser396, PHF13, 9632s, 1:1000) were obtained from Cell Signaling. GSK-3β (Clone 7, 610202, 1:1000) and Drp1 (Clone 8/DLP1, 611112, 1:1000) were obtained from BD Transduction Laboratories. Tau 5 (QF215086, 1:1000), PhosphorTau (Thr205, 44-738G, 1:2000), Phosphor-Tau (Thr214, 44-742G, 1:2000), Phosphor-Tau (Thr231, 44-746G, 1:2000) and (Thr262, 44-750G, 1:2000) were obtained from Thermo Scientific. Phosphor-Tau (Ser409, AB9662, 1:2000) was obtained from Merck Millipore. Albumin (ab106582, 1:1000) was obtained from Abcam. HA monoclonal (H9658, 1:1000) was obtained from Sigma-Aldrich. PKA RII Subunits (06-411, 1:1000) was obtained from EMD Millipore.
Phospho-GSK-3α/β (Ser21/9, 9331s, 1:1000), Phosphor-(Ser/Thr) PKA Substrate (9621s, 1:1000), and Phosphor-Tau (Ser396, PHF13, 9632s, 1:1000) were obtained from Cell Signaling. GSK-3β (Clone 7, 610202, 1:1000) and Drp1 (Clone 8/DLP1, 611112, 1:1000) were obtained from BD Transduction Laboratories. Tau 5 (QF215086, 1:1000), PhosphorTau (Thr205, 44-738G, 1:2000), Phosphor-Tau (Thr214, 44-742G, 1:2000), Phosphor-Tau (Thr231, 44-746G, 1:2000) and (Thr262, 44-750G, 1:2000) were obtained from Thermo Scientific. Phosphor-Tau (Ser409, AB9662, 1:2000) was obtained from Merck Millipore. Albumin (ab106582, 1:1000) was obtained from Abcam. HA monoclonal (H9658, 1:1000) was obtained from Sigma-Aldrich. PKA RII Subunits (06-411, 1:1000) was obtained from EMD Millipore.
Free full text: Click here
