OT-I mice and OT-I mice deficient for IL-12 receptor β1 (gifts from Dr.
Mescher, University of Minnesota) have a transgenic TCR specific for H-2Kb and OVA257–2649 (link),10 (link),73 (link). Mice were maintained under specific
pathogen-free conditions at the University of Maryland, and these studies have been
reviewed and approved by the Institutional Animal Care and Use Committee. C57BL/6 male
mice were purchased from the National Cancer Institute. Vaccinia virus preferentially
accumulates in ovaries 74 (link), 75 (link), which may cause variation in CTL activation
under different treatments. As a result, only male recipient mice were used for vaccinia
virus infection. Phospho-Stat4 (Tyr693) (D2E4) and Jak2 (D2E12) were purchased from Cell
signaling Technology (Danvers, MA). All the rest directly conjugated fluorescent
antibodies were purchased from BD Biosciences, eBioscience or Biolegend. Rapamycin were
purchased from EMD (Gibbstown, NJ). Kb/OVA tetramer is a gift from Dr. Jameson
from University of Minnesota.
Mescher, University of Minnesota) have a transgenic TCR specific for H-2Kb and OVA257–2649 (link),10 (link),73 (link). Mice were maintained under specific
pathogen-free conditions at the University of Maryland, and these studies have been
reviewed and approved by the Institutional Animal Care and Use Committee. C57BL/6 male
mice were purchased from the National Cancer Institute. Vaccinia virus preferentially
accumulates in ovaries 74 (link), 75 (link), which may cause variation in CTL activation
under different treatments. As a result, only male recipient mice were used for vaccinia
virus infection. Phospho-Stat4 (Tyr693) (D2E4) and Jak2 (D2E12) were purchased from Cell
signaling Technology (Danvers, MA). All the rest directly conjugated fluorescent
antibodies were purchased from BD Biosciences, eBioscience or Biolegend. Rapamycin were
purchased from EMD (Gibbstown, NJ). Kb/OVA tetramer is a gift from Dr. Jameson
from University of Minnesota.
