Xenopus neural crest was labelled with nuclear-RFP/membrane-GFP or membrane-RFP/nuclear-GFP. In vitro analysis of neural crest migration was performed using Xenopus neural crest cultured on fibronectin-coated plates. For in vivo studies we used Xenopus embryos grafted with labelled neural crest or zebrafish transgenic lines embryos that express cytoplasm or membrane-GFP under the neural crest promoter sox10. Time-lapse was carried out using DIC or fluorescent/confocal microscopy. FRET analysis was performed as described in14 (link). For full methods, see Supplementary Material .
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Xenopus Neural Crest Migration Assay
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Confocal microscopy
Cytoplasm
Embryos
Fibronectin
Fret
Membrane
Neural crest
Nuclear membrane
Sox10
Transgenic
Xenopus
Zebrafish
Corresponding Organization :
Other organizations : University College London, King's College London
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Variable analysis
independent variables
- Labelling method (nuclear-RFP/membrane-GFP or membrane-RFP/nuclear-GFP)
- In vitro vs in vivo studies
- Xenopus neural crest cultured on fibronectin-coated plates vs Xenopus embryos grafted with labelled neural crest vs zebrafish transgenic lines expressing GFP under the neural crest promoter sox10
- Neural crest migration
- FRET analysis
- Time-lapse using DIC or fluorescent/confocal microscopy
- Positive control: FRET analysis as described in reference 14 (https://pubmed.ncbi.nlm.nih.gov/18403410/)
- Negative control: Not specified
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