THP1 Cell Stimulation Protocol

We used the THP1 cell line for stimulation experiments. After the recovery of cells from a deep-frozen state, we used the protocol published earlier ^{45 (link),46 (link)}. The cells were stimulated for 3 h in the RPMI 1640 medium (Sigma Aldrich) containing 10% of a plasma sample. The cells were collected after 3 h of cultivation; after centrifugation, the supernatant was removed, and the cells were preserved in lysis solution (Sigma-Aldrich) and stored at − 80 °C.

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Korabecna M., Zinkova A., Brynychova I., Chylikova B., Prikryl P., Sedova L., Neuzil P, & Seda O. (2020). Cell-free DNA in plasma as an essential immune system regulator. Scientific Reports, 10, 17478.

Publication 2020

RPMI 1640 medium lysis solution

Cells Centrifugation Frozen Plasma Thp1 cell line

Corresponding Organization : General University Hospital in Prague

Other organizations : Northwestern Polytechnical University

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Variable analysis

independent variables

Stimulation of THP1 cells with a plasma sample

dependent variables

Cellular response/changes after 3 hours of stimulation

control variables

RPMI 1640 medium (Sigma Aldrich)
Plasma sample concentration (10%)
Stimulation duration (3 hours)
Storage conditions (preserved in lysis solution, stored at -80 °C)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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