Edoxaban (MedChem Express, Monmouth Junction, NJ) was used for the in vitro study. SECs were allocated to two H/R groups and then cultured (n = 5 per group). The Edoxaban and vehicle groups were pretreated with 1 μM of Edoxaban in endothelial cell medium and vehicle (0.1% DMSO equivalent to that used to dissolve Edoxaban) for 1 hour. Following the pretreatment, the SECs in both groups were exposed to 90-minute hypoxia and 4 hours reoxygenation using an AnaeroPack jar system. A dose of 1 μM had a significant effect (p = 0.008), we decided to use 1 μM of Edoxaban (S2 Fig ).
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