Evaluating Synergistic Effects of Targeted Therapies

For the case study, MCF-7 and SOX11 overexpression SH-EP cell line were grown in RPMI medium supplemented with 10% FCS and 2mM L-Glutamine and 100 IU/mL penicillin/streptomycin. The generation of SOX11 overexpression is described in [24 (link)]. To evaluate synergism and the dose-response relationship, cells were seeded in a 384-well plate (Corning COS3764), at a density of 3x10³ cells per well. Cells were allowed to adhere overnight, after which these were exposed to the respective treatment. The drugs used were: AZD2281 (Selleckchem, S1060), MK-1775 (Selleckchem, S1525), prexasertib (MedChem Express, HY-18174A) and BAY 1895344 (Selleckchem, S8666). The treatment was performed by the D300 TECAN. Cell proliferation was monitored for 72h, in which pictures were taken through IncuCyte S3 Live Cell Imaging System each 2h. Each image was analysed through the IncuCyte S3 Software. Cell proliferation was monitored by analysing the occupied area (% confluence) over time.