Dengue Virus Neutralization Assay
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Corresponding Organization : Sanofi (United States)
Other organizations : Sanofi (France)
Variable analysis
- Concentration of virus for each dengue serotype
- Concentration of 2-fold serial dilutions of sera
- Concentrations of dengue nAb in Vero cells and CV-1-Fc cells measured by PRNT
- Identical concentrations of virus for each serotype used in parallel CV-1-Fc and Vero PRNTs
- Incubation with DENV serotype-specific mouse anti-envelope mAbs and alkaline phosphatase-conjugated goat anti-mouse IgG
- Visualization of plaques using SIGMAFAST BCIP/NBT alkaline phosphatase substrate in levamisole solution
- Not explicitly mentioned
- Not explicitly mentioned
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