The genome of IMX1139 was sequenced using MiSeq (Illumina, San Diego, CA, USA) with MiSeq® Reagent Kit v3 with 2 × 300 bp read length. Genomic DNA was extracted using the Genomic DNA kit (Qiagen, Hilden, Germany). Extracted DNA was quantified by BR ds DNA kit using Qubit spectrophotometer (Invitrogen, Carlsbad, CA, USA) and mechanically sheared with the M220 ultrasonicator (Covaris, Woburn, MA, USA) using settings aiming at 550 bp average size. DNA libraries were prepared using the TruSeq DNA PCR-Free Library Preparation Kit according to the manufacturer's instructions (Illumina). qPCR quantification of libraries was done with the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems, Wilmington, MA, USA) on a Rotor-Gene Q PCR cycler (Qiagen). Sequence reads of genomic DNA were mapped onto the CEN.PK113–7D reference strain sequence (35 ) and on the unique integrated Fncpf1-KlURA3 contig using the Burrows–Wheeler Alignment tool (BWA) and further processed using SAMtools (45 (link),46 (link)). The sequencing raw data are available at NCBI (https://www.ncbi.nlm.nih.gov/bioproject/) under the Bioproject number PRJNA394199.