Primer Sequences for ChIP-qPCR and RT-qPCR Analyses
For ChIP-qPCR, primers for KLK3-enh, KLK2-enh, NKX3.1-enh, SGK1-enh, SGK3-enh, MBOAT2-enh, RAB11B-FBS, PDK4-FBS, and CDK1-FBS were listed in Supplementary Table 1. Primers for TMPRSS2-enh, ZBTB16-enh, and SH2B1-enh were described previously 11 (link). Primers for TFF1-enh, NRIP1-enh, PGR-enh, and DSCAM-enh were described previously 56 (link),57 (link). For RT-qPCR, primer and probe sets for KLK3 and ZBTB16 were listed in Supplementary Table 1. Primer and probe sets for MBOAT2 (Hs01027245_m1), ELOVL7 (Hs00405151_m1), FKBP5 (Hs01561006_m1), NKX3.1 (Hs00171834_m1), SGK1 (Hs00178612_m1), ACSL3 (Hs00244853_m1), ELOVL5 (Hs01094711_m1), NANS (Hs00219054_m1), and AR-V7 (AI6R0CI) were purchased from Thermo Fisher.
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Gao S., Chen S., Han D., Wang Z., Li M., Han W., Besschetnova A., Liu M., Zhou F., Barrett D., Luong M.P., Owiredu J., Liang Y., Ahmed M., Petricca J., Patalano S., Macoska J.A., Corey E., Chen S., Balk S.P., He H.H, & Cai C. (2020). Chromatin binding of FOXA1 is promoted by LSD1-mediated demethylation in prostate cancer. Nature genetics, 52(10), 1011-1017.
Other organizations :
University of Massachusetts Boston, University of Toronto, Princess Margaret Cancer Centre, University Health Network, University of Washington, Beth Israel Deaconess Medical Center, Harvard University
Primers for KLK3-enh, KLK2-enh, NKX3.1-enh, SGK1-enh, SGK3-enh, MBOAT2-enh, RAB11B-FBS, PDK4-FBS, and CDK1-FBS
Primers for TMPRSS2-enh, ZBTB16-enh, and SH2B1-enh
Primers for TFF1-enh, NRIP1-enh, PGR-enh, and DSCAM-enh
Primer and probe sets for KLK3 and ZBTB16
Primer and probe sets for MBOAT2, ELOVL7, FKBP5, NKX3.1, SGK1, ACSL3, ELOVL5, NANS, and AR-V7
dependent variables
Binding of transcription factors or other regulatory proteins to the specified enhancer regions (ChIP-qPCR)
Expression levels of the specified genes (RT-qPCR)
control variables
Not explicitly mentioned
controls
Positive and negative controls for ChIP-qPCR and RT-qPCR were not specified
Annotations
Based on most similar protocols
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