Soleus muscle isolation and preparation have previously been described (Moran et al., 2005 (link)). Briefly, the soleus muscle was placed in a 1.2 mL bath filled with oxygenated Krebs-Ringer buffer maintained at 25°C. The proximal muscle tendon was attached by 6–0 suture to a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc.). Measures of peak isometric, concentric, and eccentric forces during tetanic stimulation were obtained as previously described (Greising et al., 2011b (link)).
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Isolation and Measurement of Soleus Muscle
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Other organizations : College of St. Scholastica, University of Minnesota, Georgia State University, Gillette Children's Specialty Healthcare
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Variable analysis
independent variables
- None explicitly mentioned
- Peak isometric force
- Peak concentric force
- Peak eccentric force
- Temperature of Krebs-Ringer buffer (25°C)
- Oxygen level in Krebs-Ringer buffer (oxygenated)
- Muscle preparation (soleus muscle placed in 1.2 mL bath)
- Muscle attachment (proximal muscle tendon attached by 6–0 suture to a dual-mode muscle lever system)
- None explicitly mentioned
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