Protocol detail

EEG Analysis of Alcohol Withdrawal

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At postnatal day 23, surgical screws were inserted into the skull over the frontal lobe and the contralateral occipital lobe (Weinberg et al., 2013 (link)). Subsequently, wires from a pin connector strip were attached to the screws, and the connector strip was secured to the skull with cranioplastic cement. One to two days later, 2 rats were switched to control liquid diet for two days followed by alcohol diet for the next 15 days while 2 control rats remained on CD throughout the experiment. On the day of withdrawal, EPOCH wireless transmitters (Ripple, Salt Lake City, UT) were attached to the pin connector strip. Rats were placed in new cages on an EPOCH wireless receiver tray (Ripple, Salt Lake City, UT) attached to Powerlab 8/30 (ADInstruments, Colorado Springs, CO). EEG data was recorded and analyzed using Chart5 software (ADInstruments, Colorado Springs, CO).

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Harper K.M., Knapp D.J, & Breese G.R. (2015). Withdrawal from chronic alcohol induces a unique CCL2 mRNA increase in adolescent but not adult brain—relationship to blood alcohol levels and seizures. Alcoholism, clinical and experimental research, 39(12), 2375-2385.

Publication 2015

Powerlab 8/30 Chart5 software

Alcohol Cement Diet Epoch Frontal lobe Occipital lobe Rats Salt Skull Springs Surgical

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Variable analysis

independent variables

Alcohol diet
Control liquid diet

dependent variables

EEG data

control variables

Surgical screws inserted into the skull over the frontal lobe and the contralateral occipital lobe
Wires from a pin connector strip attached to the screws
Connector strip secured to the skull with cranioplastic cement
EPOCH wireless transmitters attached to the pin connector strip
Rats placed in new cages on an EPOCH wireless receiver tray attached to Powerlab 8/30

positive controls

2 control rats remained on CD throughout the experiment

negative controls

Not mentioned

Annotations

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