Microbiome Analysis of Caecal Contents

The extraction of bacterial DNA from the samples of caecal contents was performed as described previously34 (link). The hypervariable V1–V2 region of the 16 S gene was amplified by PCR with barcoded 27Fmod (5′-AGRGTTTGATYMTGGCTCAG-3′) and reverse primer 338R35 (link) (5′-TGCTGCCTCCCGTAGGAGT-3′) and then sequenced using the 454 GS FLX Titanium or 454 GS Junior system (Roche Applied Science). Operational taxonomic unit (OTU) clustering, taxonomy assignment and UniFrac analysis were performed as described previously36 (link).

Free full text: Click here

Aoki R., Kamikado K., Suda W., Takii H., Mikami Y., Suganuma N., Hattori M, & Koga Y. (2017). A proliferative probiotic Bifidobacterium strain in the gut ameliorates progression of metabolic disorders via microbiota modulation and acetate elevation. Scientific Reports, 7, 43522.

Publication 2017

454 GS FLX Titanium 454 GS Junior system

Bacterial dna Caecal Gene Primer Titanium

Corresponding Organization : Ezaki Glico (Japan)

Other organizations : The University of Tokyo, Tokai University

Top 5 similar protocols

Variable analysis

independent variables

Bacterial DNA extraction from caecal content samples

dependent variables

Amplification of the hypervariable V1–V2 region of the 16S gene by PCR
Sequencing of the amplified 16S gene region using the 454 GS FLX Titanium or 454 GS Junior system
Operational taxonomic unit (OTU) clustering
Taxonomy assignment
UniFrac analysis

control variables

The extraction of bacterial DNA from the samples of caecal contents was performed as described previously34
Operational taxonomic unit (OTU) clustering, taxonomy assignment and UniFrac analysis were performed as described previously36

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!