Tobacco Filler Nicotine Extraction and Analysis

Tobacco filler from individual cigarettes was removed from the paper wrapper, 5 mL of 2N NaOH was added to 1.0 grams of tobacco filler in a 60 mL amber vial, followed by extraction with 50 mL of an MTBE stock that contained quinoline as an internal standard. A 1 mL aliquot of the extract was placed in a 2 mL amber vial for GC/MS analysis. The sample preparation and nicotine analytical method have been previously described.¹³ Five replicates were analyzed for each variety.

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Richter P., Steven P.R., Bravo R., Lisko J.G., Damian M., Gonzalez-Jimenez N., Gray N., Keong L.M., Kimbrell J.B., Kuklenyik P., Lawler T.S., Lee G.E., Mendez M., Perez J., Smith S., Tran H., Tyx R, & Watson C.H. (2016). Characterization of SPECTRUM Variable Nicotine Research Cigarettes. Tobacco regulatory science, 2(2), 94-105.

Publication 2016

Amber Gc ms Mtbe Nicotine Quinoline Tobacco

Corresponding Organization :

Other organizations : Centers for Disease Control and Prevention, Analytical Services, Battelle, Oak Ridge Associated Universities

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Protocol cited in 11 other protocols

Variable analysis

independent variables

Tobacco filler from individual cigarettes

dependent variables

Nicotine concentration

control variables

2N NaOH concentration
MTBE stock with quinoline as an internal standard
Aliquot volume for GC/MS analysis

notes

Independent variables not explicitly mentioned.
Positive and negative controls not specified.

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