Screening FDA-Approved Drugs in Zebrafish Model

At 5 dpf, zebrafish larvae were incubated in treatment solutions for 3 h prior to imaging, and for 3 h during imaging, using 96-well ProxiPlates (PerkinElmer, 6006290). Zebrafish larvae were treated with 190 FDA-approved compounds using a Tocris small-molecule library (Tocris Bioscience, Cat. No. 7200). The library contained 10 mM stocks dissolved in dimethyl sulfoxide (DMSO), which we diluted 1000 × in egg water to a 10 µM final concentration. The imaging experiments also included untreated larvae in egg water and larvae treated with 1 µl/ml DMSO as a vehicle control. The effects of FDA-approved drugs were compared to previously obtained results^{26 (link)}, with 10 µM cyclosporine A (CsA, Enzo Life Sciences), 1 µM tacrolimus (FK506, Enzo Life Sciences), 1 µM rapamycin (Santa Cruz Biotechnology) and 5 and 10 µM proINDY (Tocris Bioscience).