The constructs used for mouse injection, including pT3-EF1α, pT3-EF1α-HA-myr-AKT (mouse), pT3-EF1α-YapS127A (human), and pCMV/sleeping beauty transposase (pCMV/SB) plasmids, were described previously35 (link)–37 (link). Plasmids were purified using the Endotoxin free Maxi Prep Kit (Sigma-Aldrich, St. Louis, MO) before being injected into mice. PD0325901 (PD901) and MLN0128 were purchased from LC Laboratories (Woburn, MA). PD901 and MLN0128 were dissolved in DMSO for in vitro experiments.
Wang P., Song X., Utpatel K., Shang R., Yang Y.M., Xu M., Zhang J., Che L., Gordan J., Cigliano A., Seki E., Evert M., Calvisi D.F., Hu X, & Chen X. (2019). MEK inhibition suppresses K-Ras wild-type cholangiocarcinoma in vitro and in vivo via inhibiting cell proliferation and modulating tumor microenvironment. Cell Death & Disease, 10(2), 120.
Other organizations :
China Agricultural University, University of Regensburg, University of California, San Francisco, Cedars-Sinai Medical Center, Universität Greifswald
Plasmids were purified using the Endotoxin free Maxi Prep Kit (Sigma-Aldrich, St. Louis, MO) before being injected into mice
PD901 and MLN0128 were dissolved in DMSO for in vitro experiments
positive controls
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negative controls
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