Perinatal Influences on DNA Methylation

Saliva is sampled using the DNA OG-575 kit (DNAGenotek, Ottawa, Ontario, Canada). DNA extraction is performed using published methods,36 (link) and DNA methylation (DNAm) analyses are carried out at the Genetics Core of the Edinburgh Clinical Research Facility (Edinburgh, UK) using Illumina Infinium MethylationEPIC (San Diego, California, USA), with interrogation of the arrays against ~850 k methylation sites. We will investigate perinatal influences on DNAm using principal component analysis, mediation and correlation analyses.

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Boardman J.P., Hall J., Thrippleton M.J., Reynolds R.M., Bogaert D., Davidson D.J., Schwarze J., Drake A.J., Chandran S., Bastin M.E, & Fletcher-Watson S. (2020). Impact of preterm birth on brain development and long-term outcome: protocol for a cohort study in Scotland. BMJ Open, 10(3), e035854.

Publication 2020

DNA OG-575 kit Infinium MethylationEPIC

Dna methylation Methylation Saliva

Corresponding Organization : MRC Centre for Reproductive Health

Other organizations : Centre for Inflammation Research

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Variable analysis

independent variables

Perinatal influences

dependent variables

DNA methylation (DNAm) levels

control variables

DNA extraction is performed using published methods
DNA methylation (DNAm) analyses are carried out at the Genetics Core of the Edinburgh Clinical Research Facility (Edinburgh, UK) using Illumina Infinium MethylationEPIC (San Diego, California, USA)

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