PFOS/PFOA Analysis in Whole Blood and Seminal Plasma

Details for the analysis of PFOS/PFOA in whole blood were previously reported [29 (link),35 (link)]. Chemical analysis of seminal plasma was performed following the same procedure adopted for whole blood and yet tested for these and other body fluids [15 (link)]. Briefly, the samples were homogenized and extracted with methyl tert-butyl ether (MTBE, J.T. Baker, Center Valley, PA, USA). The solvent was evaporated under nitrogen and replaced with methanol (J.T. Baker). Quantification was performed in a HPLC (equipped with Betasil^© C18 column, Thermo Electron Corporation, San Jose, CA, USA) interfaced to a mass spectrometer at linear triple quadrupoles, by electrospray ionization (ESI) source, working in negative ion mode (Finnigan LTQ Thermo Electron Corporation, San Jose, CA, USA). The limit of detection (LOD) for both PFOS and PFOA were 0.4 ng/mL, corresponding to the value of the compounds in the blanks +3 Standard Deviation (SD).

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La Rocca C., Tait S., Guerranti C., Busani L., Ciardo F., Bergamasco B., Perra G., Mancini F.R., Marci R., Bordi G., Caserta D., Focardi S., Moscarini M, & Mantovani A. (2015). Exposure to Endocrine Disruptors and Nuclear Receptors Gene Expression in Infertile and Fertile Men from Italian Areas with Different Environmental Features. International Journal of Environmental Research and Public Health, 12(10), 12426-12445.

Publication 2015

Betasil© C18 column Finnigan LTQ

Blood Body fluids Electron Hplc Methanol Mtbe Nitrogen Seminal plasma Solvent

Corresponding Organization :

Other organizations : Istituto Superiore di Sanità, University of Siena, Sapienza University of Rome, University of Ferrara

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Concentrations of PFOS and PFOA in whole blood and seminal plasma

control variables

Extraction method (homogenization and MTBE extraction) used for both whole blood and seminal plasma samples
HPLC-MS/MS analytical method, including the use of Betasil C18 column and Finnigan LTQ mass spectrometer in negative ion mode
Limit of detection (LOD) for both PFOS and PFOA set at 0.4 ng/mL

controls

Blanks were used to determine the limit of detection (LOD) for PFOS and PFOA, defined as the value of the compounds in the blanks +3 Standard Deviation (SD)

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