Piezo Mechanosensitive Ion Channels in Mice

All animal experiments were carried out according to protocols approved by the Harvard Medical School Institutional Animal Care and Use Committee. Mice described in the literature and purchased from the Jackson Laboratories: Piezo1^P1-tdT (stock# 029214) (Ranade et al., 2014 (link)), Piezo2-EGFP-IRES-Cre (Piezo2^{tm1.1(cre)Apat}, stock# 027719) (Woo et al., 2014 (link)), Piezo1^f/f (stock# 029213) (Cahalan et al., 2015 (link)), Piezo2^f/f (stock# 027720) (Woo et al., 2014 (link)), Prrx1-Cre (stock# 005584) (Logan et al., 2002 (link)), Sp7-GFP::Cre (stock# 006361) (Rodda and McMahon, 2006 (link)), Polr2a^{Tn(pb-CAG-GCaMP5g,-tdTomato)Tvrd} (PC::G5-tdT, stock# 024477) (Gee et al., 2014 (link)), Ai9 (Rosa26-TdTomato, stock# 007909). For embryos or neonatal mice, both male and female were used in the analyses as sex could not be clearly identified in embryos or neonatal mice. Sex-matched littermate mice were compared in postnatal mice at day 21. Three or more littermate groups were examined and representative images are shown.

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Zhou T., Gao B., Fan Y., Liu Y., Feng S., Cong Q., Zhang X., Zhou Y., Yadav P.S., Lin J., Wu N., Zhao L., Huang D., Zhou S., Su P, & Yang Y. (2020). Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin. eLife, 9, e52779.

Publication 2020

Piezo2-EGFP-IRES-Cre Prrx1-Cre PC::G5-tdT

Embryos Female Institutional animal care and use committee Ires Male Mice Neonatal Prrx1 Tdtomato

Corresponding Organization :

Other organizations : Harvard Stem Cell Institute, The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, Sun Yat-sen Memorial Hospital, Southern Medical University, Nanfang Hospital, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Brigham and Women's Hospital

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Variable analysis

independent variables

Piezo1 gene
Piezo2 gene
Prrx1-Cre
Sp7-GFP::Cre
Polr2a gene

dependent variables

Not explicitly mentioned

control variables

Sex-matched littermate mice
Three or more littermate groups

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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