Yeast One-Hybrid Assay for Transcription Factors

Yeast one-hybrid experiment was carried out according to the method described previously [41 (link)]. To generate AD-GbBM and AD-GhBM, the full-length GbBM and GhBM were amplified and cloned into the vector pJG4-5 vector (Clontech). To generate GbBMp::LacZ and GhBMp::LacZ reporter constructs, the promoter fragments were amplified and then separately subcloned into the pLacZi2μ vector. Primers were listed in Table S3. Transformants were cultivated on proper drop-out plates containing X-gal for blue color show. Representative data was shown from one of the three biological replicates which yield similar results.