To allow comparison of the effects of CBZ in different bone microenvironments, studies were performed in animal models of different strain, sex and age as follows: 1) 6-week old male BALB/c nude mice, 2) 6-week old female BALB/c nude mice (both Charles River, UK), 3) 8–9-week old female genetically engineered mice expressing GFP-positive cells of the osteoblastic lineage on a BALB/c nude background ((BALB/cAnNCrl.Cg-Tg(Col1a1-GFP)Row Foxn1nu/nu, described in [34] (link), heterozygous, referred to as GFP Ob+ mice) and 4) 17-week old female GFP Ob+ mice (both Leeds Institute for Molecular Medicine, UK).
In murine models, bone remodelling is reduced with increasing age. We therefore established effects of CBZ in young (8–9 week old) mice with high bone turnover in addition to older (17-week old) mice with lower bone turnover.
In murine models, bone remodelling is reduced with increasing age. We therefore established effects of CBZ in young (8–9 week old) mice with high bone turnover in addition to older (17-week old) mice with lower bone turnover.
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