The pBIND plasmids expressing GAL4-tagged cyclin D1 wild type and mutations were as described previously [56 (link)]. The pcDNA3.1 plasmids encoding FLAG-tagged G9aWT and deletion mutations were obtained from Dr. Eiji Hara (Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan) [57 (link)]. The pLKO lentiviral plasmid vectors of human G9a shRNA and GLP shRNA were purchased from Sigma.
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